Recombinant Human EGFR Protein, N-His&N-GST-tagged
Product Description
Cat
IMP-4419
Official Symbol
EGFR
Product Overview
Recombinant cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Description
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo-or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Expression System
Insect cell
Species
Human
Tag
N-His&N-GST
Predicted N Terminal
Met
Form
Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Molecular Mass
The recombinant human EGFR /GST chimera consists of 780 amino acids and has a calculated molecular mass of 89.1 kDa.
Protein length
Met668-Ala1210
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 75 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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