Recombinant Human ENTPD1 Protein (Thr38-Val478), C-6×His-tagged

Product Description

Cat

IMP-9808

Official Symbol

ENTPD1

Product Overview

Recombinant human CD39/ENTPD1 protein (Thr38-Val478) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.

Description

The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.

Expression System

CHO

Species

Human

Tag

C-6×His

Predicted N Terminal

Thr38

Form

Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.

Molecular Mass

Predicted Molecular Mass: 51 kDa
SDS-PAGE: 65-90 kDa, reducing conditions

Protein length

Thr38-Val478

Bio-activity

Measured by its ability to hydrolyze the 5'-phosphate groups from the substrate adenosine-5'-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >5,000 pmol/min/μg, as measured under the described conditions.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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