Recombinant Human ENTPD1 Protein (Thr38-Val478), C-6×His-tagged
Product Description
Cat
IMP-9808
Official Symbol
ENTPD1
Product Overview
Recombinant human CD39/ENTPD1 protein (Thr38-Val478) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.
Expression System
CHO
Species
Human
Tag
C-6×His
Predicted N Terminal
Thr38
Form
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Molecular Mass
Predicted Molecular Mass: 51 kDa
SDS-PAGE: 65-90 kDa, reducing conditions
Protein length
Thr38-Val478
Bio-activity
Measured by its ability to hydrolyze the 5'-phosphate groups from the substrate adenosine-5'-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >5,000 pmol/min/μg, as measured under the described conditions.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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