Description
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Bio-activity
Measured by its binding ability in a functional ELISA. When Human EpCAM/TROP1 Affinity Purified Polyclonal Antibody is immobilized at 0.25 μg/mL (100 μL/well), it binds to Biotinylated Recombinant Human EpCAM/TROP-1 Fc Chimera Avi-tag with an ED50 of 1-6 ng/mL. Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED50 for this effect is 0.7-2.8 μg/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
