Recombinant Human EPCAM Protein (Gln24-Lys265), C-hFc-tagged
Product Description
Cat
IMP-9977
Official Symbol
EPCAM
Product Overview
Recombinant human EpCAM/TROP1 protein (Gln24-Lys265) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-hFc
Predicted N Terminal
No results obtained: Gln24 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 54 kDa (monomer)
SDS-PAGE: 60-70 kDa, under reducing conditions.
Protein length
Gln24-Lys265
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 × 10^4 cells/well are added to Recombinant Human EpCAM/TROP-1 Fc Chimera and Human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 centigrade. The ED50 for this effect is 0.7-2.8 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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