IMP-4438

EPHB1

Recombinant human EPHB1 (P54762-1) extracellular domain (Met 1-Pro 540) was expressed, with a polyhistidine tag at the C-terminus.

Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

HEK293

Human

C-His

Met 18

Lyophilized from sterile PBS, pH 7.4, 5% glycerol, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant human EPHB1 consists of 534 amino acids and has a calculated molecular mass of 60 kDa as estimated in SDS-PAGE under reducing conditions.

Met1-Pro540

< 1.0 EU/μg of the protein as determined by the LAL method

> 95 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Immobilized Human EphB1 His at 2 μg/mL (100 μL/well) can bind Human Ephrin B2 His&hFc, the EC50 of Human Ephrin B2 His&hFc is 1.5-9.0 ng/mL.

Immobilized Human EphB1 His at 2 μg/mL (100 μL/well) can bind Human Ephrin-B1 His&hFc, the EC50 of Human Ephrin-B1 His&hFc is 35-200 ng/mL.