Recombinant Human EPO Protein (Ala28-Arg193)
Product Description
Cat
IMP-9743
Official Symbol
EPO
Product Overview
Recombinant human Erythropoietin/EPO (Tissue Culture Grade) (Ala28-Arg193) without tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.
Expression System
CHO
Species
Human
Tag
Tag Free
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 21 kDa
SDS-PAGE: 37 kDa, reducing conditions
Protein length
Ala28-Arg193
Bio-activity
Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.015-0.075 units/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt at -20 to -70 centigrade as supplied.
1 month at 2-8 centigrade under sterile conditions after reconstitution.
3 months at -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 U/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Binding Activity

Calibrated against the second international reference preparation of erythropoietin. Annable, L. et al. (1972) Bull. Hlth. Org. 47:99. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.015-0.075 units/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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