Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
SDS-PAGE
Bioactivity-ELISA 1
(A) TF-1 cells cultured in 1640, stimulated with 5ng/mL EPO for 72h;
(B) Unstimulated TF-1 cells cultured in1640 for 72h.
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