Recombinant Human ERBB2 Protein, C-hFc-tagged, Biotinylated
Product Description
Cat
IMP-4546
Official Symbol
ERBB2
Product Overview
Recombinant extracellular domain (Met1-Thr652) of human ErbB2 (NP_004439.2) was fused with the Fc region of human IgG1 at the C-terminus. The purified protein was biotinylated in vitro.
Description
Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Expression System
HEK293
Species
Human
Tag
C-hFc
Predicted N Terminal
Thr 23
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human ErbB2/Fc chimera is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The monomer consists of 868 amino acids and predicts a molecular mass of 96.1 kDa.
Protein length
Met1-Thr652
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Her2/ERBB2 Protein, Human, Recombinant (hFc Tag), Biotinylated at 2 μg/mL (100 μL/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 20-110 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
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