Recombinant Human ERBB2 protein, C-His-Avi Tag, Biotinylated
Product Description
Cat
IMP-597
Official Symbol
ERBB2
Product Overview
Biotinylated Recombinant Human ERBB2 protein(NP_001005862.1 )(Thr 23-Thr 652), fused to C-His-Avi Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag, Biotinylated
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a calculated MW of 73.0 kDa. The protein migrates as 85-95 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Thr 23-Thr 652
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His,Avitag with a linear range of 0.2-6 ng/mL.

Bioactivity-SPR 1
Bioactivity-SPR

Biotinylated Human Her2, His,Avitag immobilized on SA Chip can bind Herceptin with an affinity constant of 17 pM as determined in a SPR assay.

Bioactivity-FACS 1
Bioactivity-FACS

2e5 of Anti-Her2 CAR-293 were stained with 100 μL of 1 μg/mL of Biotinylated Human Her2 Protein, His,Avitag and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
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