Recombinant Human ERBB2 protein, C-His Tag, Biotinylated

Product Description

Cat

IMP-603

Official Symbol

ERBB2

Product Overview

Biotinylated Recombinant Human ERBB2 protein(NP_001005862.1 )(Thr 23-Thr 652), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 70.2 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Thr 23-Thr 652

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His Tag, primary amine labeling, long spacer with a linear range of 0.1-3.1 ng/mL.

Bioactivity-BLI 1

Immobilized Herceptin on SA Biosensor via Biotinylated Human Her2, His Tag, primary amine labeling, long spacer, can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) with an affinity constant of 1.32 μM as determined in BLI assay.

Bioactivity-FACS 1

2e5 of Anti-Her2 CAR-293 cells were stained with 100 μL of 1μg/mL of Biotinylated Human Her2, His Tag, primary amine labeling, long spacer and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry