Recombinant Human ERBB2 protein, C-His Tag, Biotinylated

Product Description

Cat

IMP-603

Official Symbol

ERBB2

Product Overview

Biotinylated Recombinant Human ERBB2 protein(NP_001005862.1 )(Thr 23-Thr 652), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 70.2 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Thr 23-Thr 652

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His Tag, primary amine labeling, long spacer with a linear range of 0.1-3.1 ng/mL.

Bioactivity-BLI 1

Immobilized Herceptin on SA Biosensor via Biotinylated Human Her2, His Tag, primary amine labeling, long spacer, can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) with an affinity constant of 1.32 μM as determined in BLI assay.

Bioactivity-FACS 1

2e5 of Anti-Her2 CAR-293 cells were stained with 100 μL of 1μg/mL of Biotinylated Human Her2, His Tag, primary amine labeling, long spacer and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry