Recombinant Human ERBB2 Protein, C-His-tagged
Product Description
Cat
IMP-4549
Official Symbol
ERBB2
Product Overview
Recombinant human ERBB2 (NP_004439.2) (Pro489-Cys630) was expressed with a polyhistidine tag at the C-terminus.
Description
Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Expression System
HEK293
Species
Human
Tag
C-His
Predicted N Terminal
Pro 489
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human ERBB2 consists of 153 amino acids and predicts a molecular mass of 17.1 kDa. As a result of glycosylation, human ErbB2 migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.
Protein length
Pro489-Cys630
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 85 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, domain IV, His Tag) at 2 μg/mL (100 μL/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 15-70 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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