Recombinant Human ERBB2 protein(498-648), C-His Tag

Product Description

Cat

IMP-604

Official Symbol

ERBB2

Product Overview

Recombinant Human ERBB2 protein(NP_001005862.1 )(Asn 498-Ala 648), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 18.5 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. This protein has been verified not recognized by Pertuzumab in functional ELISA at ACRObiosystems.

Protein length

Asn 498-Ala 648

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human Her2 (498-648), His Tag at 1 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.1-13 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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