Recombinant Human ERBB2 protein(498-648), C-His Tag
Product Description
Cat
IMP-604
Official Symbol
ERBB2
Product Overview
Recombinant Human ERBB2 protein(NP_001005862.1 )(Asn 498-Ala 648), fused to C-His Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-His Tag
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a calculated MW of 18.5 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. This protein has been verified not recognized by Pertuzumab in functional ELISA at ACRObiosystems.
Protein length
Asn 498-Ala 648
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human Her2 (498-648), His Tag at 1 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.1-13 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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