Recombinant Human ESM1 Protein, C-His-tagged
Product Description
Cat
IMP-4471
Official Symbol
ESM1
Product Overview
Recombinant human ESM1 (NP_008967.1) (Met1-Arg184) was expressed with a C-terminal polyhistidine tag.
Description
ESM1 is a secreted protein which is produced by adipocytes. It has been noticed that ESM1 may play some role in obesity-associated vascular disease since circulating ESM-1 levels are reduced in the overweight and obese. ESM1 is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of ESM1 gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. Recently, ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF or FGF-2. In hypervascularized cancers, overexpression of endocan has been detected by immunohistochemistry using monoclonal antibodies against ESM1.
Expression System
Insect cell
Species
Human
Tag
C-His
Predicted N Terminal
Trp 20
Form
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The secreted recombinant human ESM1 consists of 176 amino acids and predicts a molecular mass of 19.6 KDa. The apparent molecular mass of the protein is approximately 23-27 KDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Arg184
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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