Recombinant Human FASLG Protein (Pro134-Leu281), N-6×His-tagged

Product Description

Cat

IMP-9668

Official Symbol

FASLG

Product Overview

Recombinant human Fas Ligand/TNFSF6 protein (Pro134-Leu281) with an N-terminal Met and 6×His tag was expressed in Chinese Hamster Ovary cell line.

Description

This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.

Expression System

CHO

Species

Human

Tag

N-6×His

Predicted N Terminal

Met

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 18 kDa
SDS-PAGE: 19 - 32 kDa, reducing conditions

Protein length

Pro134-Leu281

Bio-activity

Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.3-1.5 ng/mL in the presence of 10 μg/mL of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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