Recombinant Human FCER2 Protein (Asp48-Ser321), N-9×His-tagged

Product Description

Cat

IMP-9663

Official Symbol

FCER2

Product Overview

Recombinant human CD23/Fc epsilon RII protein (Asp48-Ser321) with an N-terminal 9-His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Expression System

Mouse myeloma cell line

Species

Human

Tag

N-9×His

Predicted N Terminal

His

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 32 kDa
SDS-PAGE: 41 kDa, reducing conditions

Protein length

Asp48-Ser321

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rhCD23 at 2 μg/mL (100 μL/well) can bind human IgE with a linear range of 0.015-1 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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