Recombinant Human FCGR1A Protein, C-His&C-AVI-tagged, Biotinylated
Product Description
Cat
IMP-4279
Official Symbol
FCGR1A
Product Overview
Recombinant extracellular domain of human CD64 (NP_000557.1) (Met 1-Thr 287) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Description
High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
Expression System
HEK293
Species
Human
Tag
C-His&C-AVI
Predicted N Terminal
Gln 16
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The secreted recombinant human CD64 consists of 307 amino acids and has a predicted molecular mass of 34.4 kDa.
Protein length
Met1-Thr287
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized human IgG1 at 2 μg/mL (100 μL/well) can bind Human CD64 His&AVI, Biotinylated, the EC50 of Human CD64 His&AVI, Biotinylated is 30-120 ng/mL.

Bioactivity-BLI 1

Using the Octet RED System, the affinity constant (Kd) of CD64 Protein, Human, Recombinant (His&AVI Tag), Biotinylated bound to Avastin was 1.35 nM.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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