Recombinant Human FCGR2A Protein (Ala36-Ile218), C-10×His-tagged

Product Description

Cat

IMP-9678

Official Symbol

FCGR2A

Product Overview

Recombinant human Fc gamma RIIA/CD32a (R167) protein (Ala36-Ile218) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Ala36

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 22 kDa
SDS-PAGE: 26-36 kDa, under reducing conditions.

Protein length

Ala36-Ile218

Bio-activity

Measured by its ability to bind human IgG with an estimated Kd <80 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its ability to bind human IgG with an estimated Kd <80 nM.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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