Recombinant Human FCGR2A Protein (Ala36-Ile218), C-Avi and 6×His-tagged, Biotinylated
Product Description
Cat
IMP-10065
Official Symbol
FCGR2A
Product Overview
Biotinylated recombinant human Fc gamma RIIA/CD32a protein (Ala36-Ile218) with Avi and 6×His tag at C-terminus was expressed in Human embryonic kidney cell.
Description
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Human
Tag
C-Avi and 6×His
Predicted N Terminal
Ala36
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 24 kDa
SDS-PAGE: 32-39 kDa, under reducing conditions.
Protein length
Ala36-Ile218
Bio-activity
Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 5.00 μg/mL (100 μL/well), Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein binds with an ED50 of 1.00-8.00 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 5.00 μg/mL (100 μL/well), Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein binds with an ED50 of 1.00-8.00 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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