Recombinant Human FCGR2A Protein, C-His&C-AVI-tagged
Product Description
Cat
IMP-1990
Official Symbol
FCGR2A
Product Overview
Recombinant extracellular domain (Met 1-Met 210) of human CD32a (P12318-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.
Description
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Human
Tag
C-His&C-AVI
Predicted N Terminal
Gln 34
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 KDa. It migrates as an approximately 32 KDa band due to glycosylation by SDS-PAGE under reducing conditions.
Protein length
Met1-Met210
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 98 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Human, Recombinant (His&AVI Tag) bound to Herceptin was 1.5 nM.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
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    1-516-512-3133
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