Recombinant Human FCGR2A Protein (Gln34-Ile218), C-10×His-tagged

Product Description

Cat

IMP-9974

Official Symbol

FCGR2A

Product Overview

Recombinant human Fc gamma RIIA/CD32a (H167) protein (Gln34-Ile218) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Ala36 identified. Gln34 inferred from enzymatic pyroglutamate treatment revealing Ala35

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 22 kDa
SDS-PAGE: 25-37 kDa, reducing conditions

Protein length

Gln34-Ile218

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RIIA/CD32a (H167) is coated at 1 μg/mL, 100 μL/well, it binds to human IgG with an ED50 of 0.25-1.25 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RIIA/CD32a (H167) is coated at 1 μg/mL, 100 μL/well, it binds to human IgG with an ED50 of 0.25-1.25 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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