Recombinant Human FCGR2A Protein (Gln34-Ile218), C-10×His-tagged
Product Description
Cat
IMP-9974
Official Symbol
FCGR2A
Product Overview
Recombinant human Fc gamma RIIA/CD32a (H167) protein (Gln34-Ile218) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-10×His
Predicted N Terminal
Ala36 identified. Gln34 inferred from enzymatic pyroglutamate treatment revealing Ala35
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 22 kDa
SDS-PAGE: 25-37 kDa, reducing conditions
Protein length
Gln34-Ile218
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RIIA/CD32a (H167) is coated at 1 μg/mL, 100 μL/well, it binds to human IgG with an ED50 of 0.25-1.25 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RIIA/CD32a (H167) is coated at 1 μg/mL, 100 μL/well, it binds to human IgG with an ED50 of 0.25-1.25 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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