Recombinant Human FCGR2A(R167) protein, C-His-Avi Tag, Biotinylated
Product Description
Cat
IMP-654
Official Symbol
FCGR2A
Product Overview
Biotinylated Recombinant Human FCGR2A protein(Accession # P12318-1 (R167) )(Ala 36-Ile 218), fused to C-His-Avi Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-His-Avi Tag, Biotinylated
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a calculated MW of 24.0 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Ala 36-Ile 218
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Loaded Biotinylated Human CD32a (R167), Avitag,His Tag (SPR & BLI verified) on SA Biosensor, can bind Rituximab with an affinity constant of 0.9 μM as determined in BLI assay.

Bioactivity-SPR 1
Bioactivity-SPR

Biotinylated Human CD32a (R167), Avitag,His Tag (SPR & BLI verified) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 2.15 μM as determined in a SPR assay.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info