Recombinant Human FCGR2B protein, C-His Tag

Product Description

Cat

IMP-655

Official Symbol

FCGR2B

Product Overview

Recombinant Human FCGR2B protein(Accession # P31994)(Ala 46-Pro 217), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 20.4 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Protein length

Ala 46-Pro 217

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-BLI 1

Loaded Human CD32b, His Tag (SPR & BLI verified) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 4.30 μM as determined in BLI assay.

Bioactivity-BLI 2

Loaded Rituximab on FAB2G Biosensor, can bind Human CD32b, His Tag (SPR & BLI verified) with an affinity constant of 5.4 μM as determined in BLI assay.

Bioactivity-SPR 1

Immobilized Human CD32b, His Tag (SPR & BLI verified) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 10.1 μM as determined in a SPR assay.

Bioactivity-SPR 2

Immobilized Rituximab on CM5 Chip, can bind Human CD32b, His Tag (SPR & BLI verified) with an affinity constant of 5.5 μM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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