Recombinant Human FCGR2B Protein, C-His-tagged
Product Description
Cat
IMP-4205
Official Symbol
FCGR2B
Product Overview
Recombinant (Ala46-Pro217) of human CD32b (NP_001002274.1) was expressed with a C-terminal polyhistidine tag.
Description
The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene.
Expression System
CHO
Species
Human
Tag
C-His
Predicted N Terminal
Ala 46
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The secreted recombinant human CD32b consists of 183 amino acids and predicts a molecular mass of 20.8 KDa. The apparent molecular mass of the protein is approximately 22-29 KDa in SDS-PAGE under reducing conditions due to different glycosylation.
Protein length
Ala46-Pro217
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 97 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized human sCD32b-His (CHO) at 10 μg/mL (100 μL/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.18-0.42 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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