Recombinant Human FCGR2B Protein, C-His-tagged

Product Description

Cat

IMP-4205

Official Symbol

FCGR2B

Product Overview

Recombinant (Ala46-Pro217) of human CD32b (NP_001002274.1) was expressed with a C-terminal polyhistidine tag.

Description

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene.

Expression System

CHO

Species

Human

Tag

C-His

Predicted N Terminal

Ala 46

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The secreted recombinant human CD32b consists of 183 amino acids and predicts a molecular mass of 20.8 KDa. The apparent molecular mass of the protein is approximately 22-29 KDa in SDS-PAGE under reducing conditions due to different glycosylation.

Protein length

Ala46-Pro217

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized human sCD32b-His (CHO) at 10 μg/mL (100 μL/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.18-0.42 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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