Recombinant Human FCGR3A(F176) protein, C-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-640

Official Symbol

FCGR3A

Product Overview

Biotinylated Recombinant Human FCGR3A protein(Accession # P08637-1)(Gly 17-Gln 208), fused to C-His-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 25.5 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gly 17-Gln 208

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-BLI 1

Loaded Biotinylated Human CD16a (F176), Avitag,His Tag (SPR & BLI verified) on SA Biosensor, can bind Rituximab with an affinity constant of 1.29 μM as determined in BLI assay.

Bioactivity-SPR 1

Biotinylated Human CD16a (F176), Avitag,His Tag (SPR & BLI verified) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 1.72 μM as determined in a SPR assay.

Bioactivity-SPR 2

Immobilized Biotinylated Human CD16a (F176), Avitag,His Tag (SPR & BLI verified) on SA Chip can bind Rituximab with an affinity constant of 1.33 μM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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