Recombinant Human FCGR3A(V176) protein, C-His Tag
Product Description
Cat
IMP-643
Official Symbol
FCGR3A
Product Overview
Recombinant Human FCGR3A protein(Accession # P08637-1)(Gly 17-Gln 208), fused to C-His Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-His Tag
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a calculated MW of 23.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Gly 17-Gln 208
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by reduced SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Loaded Human CD16a (V176), His Tag (SPR & BLI verified) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay.

Bioactivity-BLI 2
Bioactivity-BLI

Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (V176), His Tag (SPR & BLI verified) with an affinity constant of 0.37 μM as determined in BLI assay.

Bioactivity-SPR 1
Bioactivity-SPR

Immobilized Human CD16a (V176), His Tag (SPR & BLI verified) on CM5 Chip via anti-His antibody, can bind Herceptin with an affinity constant of 163 nM as determined in SPR assay.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
  • 1-631-938-8127
Email
Address
Copyright © 2025 Creative BioMart. All rights reserved.

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies
x