Recombinant Human FCGR3A(V176) protein, C-His Tag

Product Description

Cat

IMP-643

Official Symbol

FCGR3A

Product Overview

Recombinant Human FCGR3A protein(Accession # P08637-1)(Gly 17-Gln 208), fused to C-His Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The protein has a calculated MW of 23.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Gly 17-Gln 208

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-BLI 1

Loaded Human CD16a (V176), His Tag (SPR & BLI verified) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay.

Bioactivity-BLI 2

Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (V176), His Tag (SPR & BLI verified) with an affinity constant of 0.37 μM as determined in BLI assay.

Bioactivity-SPR 1

Immobilized Human CD16a (V176), His Tag (SPR & BLI verified) on CM5 Chip via anti-His antibody, can bind Herceptin with an affinity constant of 163 nM as determined in SPR assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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