Recombinant Human FCGR3B Protein (Thr20-Gln208), C-10×His-tagged

Product Description

Cat

IMP-9693

Official Symbol

FCGR3B

Product Overview

Recombinant human Fc gamma RIIIB/CD16b protein (Thr20-Gln208) with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-10×His

Predicted N Terminal

Thr20

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 22.7 kDa
SDS-PAGE: 40-60 kDa, reducing conditions

Protein length

Thr20-Gln208

Bio-activity

Measured by its ability to bind human IgG with an estimated Kd <150 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its ability to bind human IgG with an estimated Kd <150 nM.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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