Recombinant Human FCGR3B(NA1) protein, N-His-SUMO Tag
Product Description
Cat
IMP-649
Official Symbol
FCGR3B
Product Overview
Recombinant Human FCGR3B protein(Accession # O75015)(Gly 17-Ser 200), fused to N-His-SUMO Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
N-His-SUMO Tag
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The protein has a calculated MW of 33.6 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE).
Protein length
Gly 17-Ser 200
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SEC-MALS 1
Bioactivity-BLI 1
Loaded Human CD16b (NA1), SUMO,His Tag on HIS1K Biosensor, can bind Rituximab with an affinity constant of 8.3 μM as determined in BLI assay.