Recombinant Human FCGRT&B2M protein, C-His-Avi Tag, Biotinylated

Product Description

Cat

IMP-638

Official Symbol

FCGRT

Product Overview

Biotinylated Recombinant Human FCGRT&B2M protein(Accession # P55899-1 & P61769-1)(Ala 24-Ser 297 (FCGRT) & Ile 21-Met 119 (B2M)), fused to C-His-Avi Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His-Avi Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The reducing (R) protein migrates as 38-40 kDa (FCGRT) and 12 kDa (B2M) respectively due to glycosylation.

Protein length

Ala 24-Ser 297 (FCGRT) & Ile 21-Met 119 (B2M)

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-BLI 1

Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag on SA Biosensor, can bind Human Serum Albumin, His Tag with an affinity constant of 0.647 μM as determined in BLI assay.

Bioactivity-SPR 1

Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag captured on Biotin CAP-Series S sensor Chip can bind Herceptin with an affinity constant of 0.11 μM as determined in a SPR assay.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

Online Inquiry