Recombinant Human FCGRT&B2M protein, C-His & C-Strep II Tag, Biotinylated

Product Description

Cat

IMP-639

Official Symbol

FCGRT

Product Overview

Biotinylated Recombinant Human FCGRT&B2M protein(Accession # P55899-1 & P61769-1)(Ala 24-Ser 297 (FCGRT) & Ile 21-Met 119 (B2M)), fused to C-His & Strep II Tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

C-His & Strep II Tag, Biotinylated

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Molecular Mass

The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

Protein length

Ala 24-Ser 297 (FCGRT) & Ile 21-Met 119 (B2M)

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

SDS-PAGE

Bioactivity-BLI 1

Loaded Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling on SA Biosensor, can bind Herceptin with an affinity constant of 0.36 μM as determined in BLI assay.

Bioactivity-BLI 2

Loaded Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling on SA Biosensor, can bind Human Serum Albumin, His Tag with an affinity constant of 0.263 μM as determined in BLI assay.

Bioactivity-SPR 1

Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling captured on Biotin CAP-Series S sensor Chip can bind Herceptin with an affinity constant of 0.57 μM as determined in a SPR assay (Biacore 8K).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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