Recombinant Human FCGRT&B2M protein, C-His Tag
Product Description
Cat
IMP-637
Official Symbol
FCGRT
Product Overview
Recombinant Human FCGRT&B2M protein(Accession # P55899-1 & P61769-1)(Ala 24-Ser 297 (FCGRT) & Ile 21-Met 119 (B2M)), fused to C-His Tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-His Tag
Form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass
The reducing (R) protein migrates as 33 kDa (FCGRT) and 10 kDa (B2M) respectively due to glycosylation.
Protein length
Ala 24-Ser 297 (FCGRT) & Ile 21-Met 119 (B2M)
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.14 μM as determined in BLI assay.

Bioactivity-SPR 1
Bioactivity-SPR

Captured Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) on CM5 Chip via Anti-His antibody can bind Herceptin (Trastuzumab) with an affinity constant of 0.516 μM as determined in a SPR assay.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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