Recombinant Human FCGRT&B2M Protein, C-His-tagged, Biotinylated

Product Description

Cat

IMP-4488

Official Symbol

FCGRT&B2M

Product Overview

Recombinant extracellular domain (Met1-Ser297) of human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified protein was biotinylated in vitro.

Description

This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Ala 24&Ile 21

Form

Lyophilized from sterile PBS, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of human FCGRT/B2M consists of 385 (285 + 100) amino acids and predicts a molecular mass of 43.5 (31.8 + 11.7) kDa.

Protein length

Met1-Ser297 and Met1-Met119

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-BLI 1

Using the Octet RED System, the affinity constant (Kd) of FCGRT&B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated bound to IgG1 Antibody was 4.6 nM.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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