Recombinant Human FCGRT&B2M Protein, C-His&C-AVI-tagged, Biotinylated

Product Description

Cat

IMP-4489

Official Symbol

FCGRT&B2M

Product Overview

Recombinant extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; Recombinant human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Description

This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants.

Expression System

HEK293

Species

Human

Tag

C-His&C-AVI

Predicted N Terminal

Ala 24&Ile 21

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of human FCGRT/B2M comprises 399 (300+99) amino acids and has a calculated molecular mass of 45.3 (33.6+11.7) KDa.

Protein length

Met1-Ser297 and Met1-Met119

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-BLI 1

Using the Octet RED System, the affinity constant (Kd) of FCGRT&B2M Heterodimer Protein, Human, Recombinant (AVI Tag), Biotinylated bound to Herceptin was 0.03 μM.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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