Recombinant Human FGFR3 Protein (Glu23-Gly377), C-6×His and Avi-tagged, Biotinylated
Product Description
Cat
IMP-10058
Official Symbol
FGFR3
Product Overview
Biotinylated recombinant human FGFR3 (IIIb) protein (Glu23-Gly377) with 6×His tag and Avi tag at C-terminus was expressed in Human embryonic kidney cell.
Description
This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.
Expression System
HEK293
Species
Human
Tag
C-6×His and Avi
Predicted N Terminal
Glu23
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 41 kDa
SDS-PAGE: 55-75 kDa, under reducing conditions.
Protein length
Glu23-Gly377
Bio-activity
Measured by its binding ability in a functional ELISA. In a Human FGF acidic/FGF1 antibody coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1, Biotinylated Recombinant Human FGFR3 (IIIb) His-tag Avi-tag Protein binds with an ED50 of 1.00-6.00 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. In a Human FGF acidic/FGF1 antibody coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1, Biotinylated Recombinant Human FGFR3 (IIIb) His-tag Avi-tag Protein binds with an ED50 of 1.00-6.00 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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