Recombinant Human FLT1 protein, C-hFc tag
Product Description
Cat
IMP-661
Official Symbol
FLT1
Product Overview
Recombinant Human FLT1(P17948)(Ser27-Asn756) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Human
Tag
C-hFc tag
Form
Lyophilized from sterile PBS, pH 7.4.
Molecular Mass
The protein has a calculated MW of 109.3kDa, and migrates as 150-160 kDa under reducing conditions.
Protein length
Ser27-Asn756
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-Elisa

Immobilized Human VEGF165 at 2μg/ml (100 μl/well) can bind Human VEGFR1/FLT1, The ED50 of Human VEGFR1/FLT1 is 36.4 ng/ml.

Bioactivity-BLI 1

Loaded Human VEGFR1-Fc on Protein A Biosensor, can bind Human VEGF165 with an affinity constant of 0.29 nM as determined in BLI assay.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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