Recombinant Human FLT1 protein, C-hFc tag

Product Description

Cat

IMP-661

Official Symbol

FLT1

Product Overview

Recombinant Human FLT1(P17948)(Ser27-Asn756) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.

Expression System

HEK293

Species

Human

Tag

C-hFc tag

Form

Lyophilized from sterile PBS, pH 7.4.

Molecular Mass

The protein has a calculated MW of 109.3kDa, and migrates as 150-160 kDa under reducing conditions.

Protein length

Ser27-Asn756

Endotoxin

Less than 1.0 EU per ug as determined by the LAL method.

Purity

>95% as determined by reducing SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Bioactivity-ELISA 1

Immobilized Human VEGF165 at 2μg/ml (100 μl/well) can bind Human VEGFR1/FLT1, The ED50 of Human VEGFR1/FLT1 is 36.4 ng/ml.

Bioactivity-BLI 1

Loaded Human VEGFR1-Fc on Protein A Biosensor, can bind Human VEGF165 with an affinity constant of 0.29 nM as determined in BLI assay.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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