Product Overview
Recombinant human Fibronectin Fragment 3 protein, Glu1266-Pro1908 (Met1874Thr), with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. When 5 × 10^4 cells/well are added to Recombinant Human Fibronectin Fragment 3/amino acids
1266-1908 coated plates (1 μg/mL with 100 μL/well), approximately 50-75% will adhere after 30-60 minutes at 37 centigrade.
Optimal concentration depends on cell type as well as the application or research objective.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
