Product Overview
Recombinant human Fibronectin protein (Met1-Pro1908) with a C-terminal 6×His tag was expressed in Spodoptera frugiperda, Sf 9 (baculovirus) cells and was produced in an animal component free process (ACFP).
Description
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Bio-activity
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1-5 μg/cm^2.
Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 μg/mL.
Optimal concentrations will need to be determined for individual user applications.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
