Recombinant Human FN1 Protein (Ser607-Pro1265), C-6×His-tagged
Product Description
Cat
IMP-10133
Official Symbol
FN1
Product Overview
Recombinant human Fibronectin Fragment 2 protein (Ser607-Pro1265) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
Ser607
Form
Lyophilized from a 0.2 μm filtered solution in PBS and Tween 20.
Molecular Mass
Predicted Molecular Mass: 73 kDa
SDS-PAGE: 100 kDa, reducing conditions
Protein length
Ser607-Pro1265
Bio-activity
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25-1.25 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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