Recombinant Human FZD8 Protein (Ala25-Pro172), C-Fc-tagged, Biotinylated conjugated
Product Description
Cat
IMP-10139
Official Symbol
FZD8
Product Overview
Biotinylated recombinant human Frizzled-8 protein with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.
Description
This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.
Expression System
CHO
Species
Human
Tag
C-Fc
Predicted N Terminal
Ala25
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 43 kDa
SDS-PAGE: 56-63 kDa, under reducing conditions
Protein length
Ala25-Pro172
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Wnt-3a is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED50 of 0.5-3 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer, biotinylated via amines
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Wnt-3a is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED50 of 0.5-3 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
Phone
  • 1-631-559-9269
    1-516-512-3133
Fax
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