Recombinant Human GAS6(Q14393)(Ala31-Ala678) protein, fused with a Fc tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Human
Tag
C-hFc tag
Form
Lyophilized from sterile PBS, pH 7.4.
Molecular Mass
The protein has a calculated MW of 98.8kDa, and migrates as 110-120kDa under reducing conditions.
Protein length
Ala31-Ala678
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
Bioactivity-ELISA 1
Immobilized Human Dtk-His at 10μg/ml (100 μl/well) can bind Human GAS6-Fc. The ED50 of Human GAS6-Fc is 0.336 ug/ml.
Bioactivity-BLI 1
Loaded Human AKL-His on HIS1K Biosensor, can bind Human GAS6-Fc with an affinity constant of 0.006 nM as determined in BLI assay.