Recombinant Human GAS6(Q14393)(Ala31-Ala678) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Human
Tag
C-His tag
Form
Liquid in sterile PBS, 10% Glycerol, pH 7.4.
Molecular Mass
The protein has a calculated MW of 72.7kDa, and migrates as 80-90kDa under reducing conditions.
Protein length
Ala31-Ala678
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Concentration
Please refer to the COA for detailed information.
SDS-PAGE
Bioactivity-ELISA 1
Immobilized Human AXL-His at 10μg/ml (100 μl/well) can bind Human GAS6-His: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human GAS6-His is 0.04466 ug/ml.