Recombinant Human GAS6(Q14393)(Ala31-Ala678) protein, fused with a 6*His tag at the C-terminus, was expressed from HEK293 Cells.
Expression System
HEK293
Species
Human
Tag
C-His tag
Form
Liquid in sterile PBS, 10% Glycerol, pH 7.4.
Molecular Mass
The protein has a calculated MW of 72.7kDa, and migrates as 80-90kDa under reducing conditions.
Protein length
Ala31-Ala678
Endotoxin
Less than 1.0 EU per ug as determined by the LAL method.
Purity
>95% as determined by reducing SDS-PAGE.
Storage
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Concentration
Please refer to the COA for detailed information.
SDS-PAGE
Bioactivity-ELISA 1
Immobilized Human AXL-His at 10μg/ml (100 μl/well) can bind Human GAS6-His: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human GAS6-His is 0.04466 ug/ml.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.