Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Western Blot 1
SDS-PAGE
Bioactivity-ELISA 1
(A)DU145 cells cultured in serum-free,stimulated with 100ng/mL Gas6 for 72h;
(B)Unstimulated DU145 cells cultured in serum-free DMEM for 96h.