Recombinant Human GGH Protein, C-His-tagged

Product Description

Cat

IMP-4534

Official Symbol

GGH

Product Overview

Recombinant human GGH (NP_003869.1) (Met1-Asp318) was expressed with a polyhistidine tag at the C-terminus.

Description

gamma-Glutamyl hydrolase (GGH) regulates intracellular folates and antifolates such as methotrexate (MTX) for proper nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. GGH is a ubiquitously-expressed enzyme that regulates intracellular folate metabolism for cell proliferation, DNA synthesis, and repair. cytoplasmic GGH might play a relevant role in the development and progression of invasive breast cancer.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Arg 25

Form

Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human GGH consists of 305 amino acids and predicts a molecular mass of 35.1 kDa.

Protein length

Met1-Asp318

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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