Recombinant Human GGH Protein, C-His-tagged

Product Description

Cat

IMP-4534

Official Symbol

GGH

Product Overview

Recombinant human GGH (NP_003869.1) (Met1-Asp318) was expressed with a polyhistidine tag at the C-terminus.

Description

gamma-Glutamyl hydrolase (GGH) regulates intracellular folates and antifolates such as methotrexate (MTX) for proper nucleotide biosynthesis and antifolate-induced cytotoxicity, respectively. GGH is a ubiquitously-expressed enzyme that regulates intracellular folate metabolism for cell proliferation, DNA synthesis, and repair. cytoplasmic GGH might play a relevant role in the development and progression of invasive breast cancer.

Expression System

HEK293

Species

Human

Tag

C-His

Predicted N Terminal

Arg 25

Form

Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant human GGH consists of 305 amino acids and predicts a molecular mass of 35.1 kDa.

Protein length

Met1-Asp318

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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