Recombinant Human GPC1 Protein (Asp24-Ser530), C-6×His-tagged

Product Description

Cat

IMP-10146

Official Symbol

GPC1

Product Overview

Recombinant human Glypican 1 protein (Asp24-Ser530) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Expression System

Mouse myeloma cell line

Species

Human

Tag

C-6×His

Predicted N Terminal

Asp24

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 56.8 kDa
SDS-PAGE: 60 kDa, reducing conditions

Protein length

Asp24-Ser530

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized rhGlypican 1 at 3 μg/mL (100 μL/well) will bind rhFGF-basic with an apparent KD < 1 nM.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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