Recombinant Human GPC3 Protein (Gln25-His559), C-6×His-tagged
Product Description
Cat
IMP-10147
Official Symbol
GPC3
Product Overview
Recombinant human Glypican 3 protein (Gln25-His559) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Human
Tag
C-6×His
Predicted N Terminal
No results obtained: Gln25 predicted, Ser359 & Val483
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 61.6 kDa
SDS-PAGE: 60-100 kDa, non-reducing conditions
Protein length
Gln25-His559
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Glypican 3 is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human FGF basic 146 aa that produces 50% of the optimal binding response is approximately 0.6-3 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Glypican 3 is subject to endoproteolytic processing by proprotein convertases (PC). By amino acid sequencing, three peptides (the first with a blocked N-terminus most likely starts with Gln25, the second peptide starts with Ser359 after a furin cleavage site, and the third peptide starts with Val483) are present in the recombinant GPC3 preparation. Peptides 2 and 3 are detected at a 1:1 ratio. All three peptides remained associated via disulfide bonds.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Glypican 3 is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human FGF basic 146 aa that produces 50% of the optimal binding response is approximately 0.6-3 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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