Immobilized Biotinylated Human GPRC5D VLP on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 20.4ng/ml determined by ELISA.
Amine coupling SA on CM5 Chip, SA capture Biotinylated Human GPRC5D VLP, Anti-GPRC5D Antibody as analyte; The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200).