Recombinant Human GUCY2C protein, C-hFc Tag
Product Description
Cat
IMP-6054
Official Symbol
GUCY2C
Product Overview
Recombinant Human GUCY2C protein(P25092)(24-430aa), fused with C-terminal hFc tag, was expressed in HEK293.
Expression System
HEK293
Species
Human
Tag
C-hFc Tag
Form
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Molecular Mass
74.9 kDa
Protein length
24-430aa
AA Sequence
SQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQ
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 94.8% as determined by SDS-PAGE.
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody, the EC50 is 3.049-4.660 ng/mL.
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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