Recombinant Human GUCY2C protein, N-His Tag

Product Description

Cat

IMP-6062

Official Symbol

GUCY2C

Product Overview

Recombinant Human GUCY2C protein(P25092)(24-430aa), fused with N-terminal 10xHis tag, was expressed in HEK293.

Expression System

HEK293

Species

Human

Tag

N-His Tag

Form

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Molecular Mass

49.6 kDa

Protein length

24-430aa

AA Sequence

SQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQ

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Purity

Greater than 95% as determined by SDS-PAGE.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody, the EC50 is 11.89-24.13 ng/mL.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 ug/ml can bind Anti-GUCY2C recombinant antibody, the EC50 is 11.89-24.13 ng/mL

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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