Recombinant Human GUCY2C Protein (Ser21-Gln430), C-Fc-tagged

Product Description

Cat

IMP-10157

Official Symbol

GUCY2C

Product Overview

Recombinant human Guanylyl Cyclase C protein (Ser21-Gln430) with a human IgG1 (Pro100-Lys330) Fc tag was expressed in human embryonic kidney cell.

Description

This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive).

Expression System

HEK293

Species

Human

Tag

C-Fc

Predicted N Terminal

Ser21

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 73 kDa
SDS-PAGE: 100-115 kDa, under reducing conditions

Protein length

Ser21-Gln430

Bio-activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.5 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 1 mg/mL in PBS.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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