Recombinant Human GUCY2C Protein (Ser24-Gln430), C-6×His and Avi-tagged, Biotinylated conjugated
Product Description
Cat
IMP-10156
Official Symbol
GUCY2C
Product Overview
Biotinylated recombinant human Guanylyl Cyclase C protein (Ser24-Gln430) with a 6×His tag and Avi-tag was expressed in human embryonic kidney cell.
Description
This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive).
Expression System
HEK293
Species
Human
Tag
C-6×His and Avi
Predicted N Terminal
Ser24
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Conjugate
Biotinylated
Molecular Mass
Predicted Molecular Mass: 49 kDa
SDS-PAGE: 75-85 kDa, under reducing conditions.
Protein length
Ser24-Gln430
Bio-activity
Measured by its binding ability in a functional ELISA. When Human Guanylyl Cyclase C Antibody is immobilized at 100 ng/mL (100 μL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag binds with an ED50 of 0.250-1.50 ng/mL. Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.50 μg/mL of protein is able to significantly inhibit neurite outgrowth.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Biotinylated via Avi-tag
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 1 mg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Human Guanylyl Cyclase C Antibody is immobilized at 100 ng/mL (100 μL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag binds with an ED50 of 0.250-1.50 ng/mL. Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.50 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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