Recombinant Human GUCY2C Protein (Ser24-Gln430), C-6×His and Avi-tagged, Biotinylated conjugated

Product Description

Cat

IMP-10156

Official Symbol

GUCY2C

Product Overview

Biotinylated recombinant human Guanylyl Cyclase C protein (Ser24-Gln430) with a 6×His tag and Avi-tag was expressed in human embryonic kidney cell.

Description

This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive).

Expression System

HEK293

Species

Human

Tag

C-6×His and Avi

Predicted N Terminal

Ser24

Form

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Conjugate

Biotinylated

Molecular Mass

Predicted Molecular Mass: 49 kDa
SDS-PAGE: 75-85 kDa, under reducing conditions.

Protein length

Ser24-Gln430

Bio-activity

Measured by its binding ability in a functional ELISA. When Human Guanylyl Cyclase C Antibody is immobilized at 100 ng/mL (100 μL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag binds with an ED50 of 0.250-1.50 ng/mL. Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.50 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Biotinylated via Avi-tag

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 1 mg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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