Recombinant Human HAVCR2 protein, C-mFc Tag
Product Description
Cat
IMP-150
Official Symbol
HAVCR2
Product Overview
Recombinant Human HAVCR2 protein(AA Ser 22-Arg 200)(Accession # Q8TDQ0-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.
Expression System
HEK293
Species
Human
Tag
C-mFc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 46.8 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Ser 22-Arg 200
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Human TIM-3, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Human TIM-3, Mouse IgG2a Fc Tag, low endotoxin at 0.2 ug/mL (100 uL/well) can bind Anti-TIM3 MAb, Human IgG4 with a linear range of 0.2-2 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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